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KMID : 0545119930030040244
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 4 p.244 ~ p.251
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Optimization of an Intact Cell System of Rhodocyclus gelatinosus KUP-74 for δ-Aminolevulinic Acid Production
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Lim, Wang-Jin
Choi, Kyung-Min/Hwang, Se Young
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Abstract
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A novel system has been developed to produce δ-aminolevulinic acid (ALA) using the intact cells of late logarithmic phase of Rhodocyclus gelatinosus KUP-74. The system was shown optimum yield of extracellular ALA under a condition of anaerobic light irradiation (4 Klux) at 30℃ with no variation in cell mass. The rate of extracellular ALA formation was stimulated by low doses of either C_4 or C_5 ALA biosynthetic precursors, where 5 mM (C_4) and 3 mM (C_5) of each precursors were appeared to generate the maximum rates of 3.3 and 4.0 nmoles of ALA per 0.35 ㎎ cells per hr, respectively. Half-life of the system was 10 hr in a sense of an ability of portage transport of L-glutamate, and sequential dose of this compound was resulted in promising recovery of the ALA.
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